Detection of Volatile Organic Compounds as Potential Novel Biomarkers for Chorioamnionitis – Proof of Experimental Models

Background: Histologic chorioamnionitis is only diagnosed postnatally which prevents interventions. We hypothesized that volatile organic compounds (VOCs) in the amniotic fluid might be useful biomarkers for and VOC profiles differ between amnionitis of different origins. Methods: Time-mated ewes received intra-amniotic injections media or saline (controls), live Ureaplasma parvum serovar 3 (Up) 14, 7 3d prior to c-section at day 124 gestational age (GA). 100 ?g recombinant ovine IL-1? was instilled 7, 1d delivery. Headspace were measured from fluids birth using ion mobility spectrometer coupled with multi-capillary columns. Results: 127 peaks identified. 27 VOCs differed samples controls Up- induced amnionitis. The best discrimination by Up vs. reached 2-methylpentane, a sensitivity/specificity 96/95% positive predictive value/negative values 96 95%. concentration 2-methylpentane peaked 7d after instillation Up. Discussion: established novel method study headspace fluids. may tool detect assess duration previously described exhalate women pre-eclampsia biomarker Amniotic analyzed spectrometry columns provide bedside diagnosis understanding inflammatory mechanisms during pregnancy.